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色谱柱家族新成员 - Halo® 1.5

HPLC Columns for Protein Analysis

HALO® BIOCLASS蛋白质色谱柱包括1000 Å Fused-Core®填料，均容许大分子能够不受限制地进入孔隙，从而为生物药的研发赋予了超快速以及高分辨的分析能力。

随着复杂生物制药的不断发展，经常需要对药物分子进行结构修饰，而这些修饰会在蛋白质链、糖基化位点和自由巯基等方面发生细微差别，从而产生变异体。

HALO® BIOCLASS 蛋白分析柱之特色：

  • 相对于较小的孔径和非核壳型的填料， 具有完全的孔表面可达性，从而使半峰宽更窄，回收率更高
  • 能够应用于UHPLC、HPLC和LC-MS等仪器
  • 优异的高温稳定性（最高可达90˚C），峰形和回收率均比同类产品要好
  • 在低温条件下可实现高回收率的、不同的键合相
bioclass for protein separations

Applications

  • mAbs
  • ADCs
  • Biosimilars
  • H/D exchange
  • Fragments

Features

  • Outstanding temperature stability up to 90 °C
  • Compatible with UHPLC, HPLC and MS
  • Elution of very large proteins with excellent peak shape and recovery
  • Very low LCMS bleed

Available Particles

1000 Å

protein

BEST USES

When the most comprehensive information is needed. Best for both characterization and release assays, as it delivers speed and efficiency.

AVAILABLE BONDED PHASES

C4, Diphenyl, ES-C18

PARTICLE SIZE

2.7 µm

TOTAL PORE ACCESS FOR HIGH RESOLUTION

total pore access for high resolution with halo 1000 a c4 column

The large pores of the HALO® 1000 Å C4 column allow improved access to the stationary phase and increased resolution for IgG2 isoforms compared to the smaller 300 Å pores of the FPP C4 column.

400 Å

protein

BEST USES

Ideal for fast release assays, delivers speed and efficiency.

AVAILABLE BONDED PHASES

C4, Diphenyl, ES-C18

PARTICLE SIZE

3.4 µm

FAST AND EFFICIENT RESOLUTION

fast and efficient resolution on protein separations with halo 400 a c protein c4 column

Very high resolution is obtained between variants of light and heavy chains of a reduced and alkylated monoclonal antibody (IgG1) sample using a HALO® 400 Å Protein C4 column.

Featured Documents

Application Notes

Technical Literature

Catalog

halo bioclass for glycan analysis, protein separation and peptide separation

Guidebook

halo columns bioanalysis solutions guidebook
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